Protocol

The table below lists protocols for the decontamination of cryostored carriers prior to warming. The protocols involve washing the frozen carrier retrieved from cryo-storage, with sterile Liquid Nitrogen, prior to warming.

The protocols offered are two; one ("3-Wash") is recommended for use with carriers that have been cryo-stored for a long time and that have a high probability of being contaminated. The other ("1-Wash") is recommended for carriers that have been prepared using sterile Liquid Nitrogen and which therefore have a low probability of being contaminated.

Decontamination of carriers using sterile Liquid Nitrogen ("3-Wash")

3-Wash is recommended for use with carriers that have been cryo-stored for a long time and that have a high probability of being contaminated.

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Decontamination of carriers using sterile Liquid Nitrogen ("1-Wash")

1-Wash is recommended for carriers that have been prepared using sterile Liquid Nitrogen and which therefore have a low probability of being contaminated.

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